EDAM - Bioscientific data analysis ontology

Last uploaded: February 26, 2021
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Preferred Name

Scaffold gap completion
Synonyms
Definitions

Fill the gaps in a sequence assembly (scaffold) by merging in additional sequences. Different techniques are used to generate gap sequences to connect contigs, depending on the size of the gap. For small (5-20kb) gaps, PCR amplification and sequencing is used. For large (>20kb) gaps, fragments are cloned (e.g. in BAC (Bacterial artificial chromosomes) vectors) and then sequenced.
ID

http://edamontology.org/operation_3217
comment

Different techniques are used to generate gap sequences to connect contigs, depending on the size of the gap. For small (5-20kb) gaps, PCR amplification and sequencing is used. For large (>20kb) gaps, fragments are cloned (e.g. in BAC (Bacterial artificial chromosomes) vectors) and then sequenced.
Created in

1.1
hasDefinition

Fill the gaps in a sequence assembly (scaffold) by merging in additional sequences.
inSubset

http://edamontology.org/bio

http://edamontology.org/operations
label

Scaffold gap completion
prefixIRI

operation_3217
prefLabel

Scaffold gap completion
subClassOf

http://edamontology.org/operation_3216
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